Measurements carried out with heating or cooling of the sample allows studying the changes of sample’s properties with the variations of temperature. There are several capabilities of carrying out the investigations with the varying temperatures in the air and in liquids in NT-MDT equipment.
Measuring in the magnetic field allows observing magnetic reversal processes and other effects that depend on the magnetic field. NT-MDT devices allow carrying out measuring in the longitudinal and perpendicular magnetic fields. The strength of the magnetic field is controlled by the build-in Hall effect sensor.
The investigation of biological objects, in particular, cells by scanning probe microscopy (SPM) demands a special preparing of biological object. This preparing means the fixation of the biological object.
The term “nanolithography” is commonly used for the local change of any properties of a surface by a scanning probe microscope (SPM) tip. It is the complex technique of creating and visualizing nanometer functional elements, including individual molecules and atoms, on a surface.
Preliminary investigations showed that the selective chemical etching is effective to study the internal structures of materials, composed of micro- and nanofragments with different etching rates.
During scanning in contact mode the cantilever bends not only along normally to the surface but also the cantilever torsional (lateral) deformation occurs. LFM measures the torsional deformation of the cantilever during scanning in contact mode.
The work demonstrates on the biotin-streptavidin case abilities of SPM to be used for investigations of single molecule interactions. With using this technique some specific cases of binding were found.
As well known the classical resolution limit of conventional optical microscopes which arise from diffraction on entrancetive objective is equal to λ/2. It is deduced from approximation of flat waves, i.e. objective situating at the wave region of object radiation and light waves which come from this object may be considered as flat waves.
Magnetic force microscopy (MFM) is effective tool to magnetic investigations on submicron scale. Image obtained by MFM is the space distribution of some parameter characterizing magnetic probe-sample interaction, i.e. interaction force, amplitude of vibrating magnetic probe etc.
Standard NT-MDT SPMs allow investigating a surface in temperature conditions from room one up to 150°C. The modified SMENA with heating stage provides the possibility of measurements with temperature up to 300°C.
Demonstration of the pseudoatomic resolution in AFM imaging on open air is performed conveniently with a testing HOPG sample. The measurement procedure needs to use a sufficiently stiff and short cantilever and to define the load so that the pseudoatomic resolution was achieved both with the LAT signal distribution and with the DFL signal distribution, with the sample remaining non-destructed.